Full Time Research Assistant (Metabolic Liver Disease Lab) Jobs, in National University Of Singapore Central Region (Singapore) - Maukerja

Job Description

Purpose of the post

The National University of Singapore invites applications for Research Assistant for the Metabolic Liver Disease Lab in the Department of Medicine, Yong Loo Lin School of Medicine. This role explores functional genomic methods to better understand the molecular mechanisms of metabolic dysfunction-associated fatty liver disease (MAFLD). The RA will get opportunity to collaborate with various teams across the School of Medicine, including pathologists, hepatologists, and data scientists. The ideal candidate will have demonstrated expertise in in vitro mammalian cell-based assays and a keen interest in uncovering the underpinning biological mechanisms of disease.

Job description:

• Assist PI/PF to develop, optimize, and validate in vitro mammalian cell-based assays that are related to metabolic diseases (such us cell culture, RNA/protein extraction qPCR, western blotting and other metabolic-related assays)

• To perform other related duties, incidental to the work described herein (such as conduct data analysis, ensure the validity and reliability of data, maintain accurate and complete records of all findings, present findings to colleagues)

• Assist in Daily lab management tasks such as inventory of supplies and samples, preparing sample processing media and solutions, autoclaving materials, ordering supplies and stocks
• Assist in Laboratory equipment management.
• Assist PI/RF with ethics/grant application

Qualifications

• Minimal education with B.Sc. and/or M.Sc. in Cell Biology, Biochemistry, Molecular Biology, Biomedical or related disciplines with research experience.
• Understanding of the molecular/cellular basis of MAFLD, obesity and T2D, e.g., lipid metabolism dysregulation, insulin resistance, inflammatory pathways) are desired.

• Experience in handling 2D and 3D cell cultures such as hepatocytes, adipocytes or skeletal muscle cells is essential.

• Basic Molecular Biology skills (RNA/protein extraction, qPCR, western blotting) is essential.

• Experience with gene perturbation tools, such as siRNA, shRNA and CRISPR/Cas9 is a strong plus.

• Excellent writing and communication skills in English.
• Highly organized and ability to independent judgement and produce a quality work product within tight time constraint.

• Ability to coordinate and work in a multidisciplinary team.

Applicants should send the following documents by email to Tan Yu Xi (NUH) <*************

and please mention “Application for RA-Metabolic Liver Disease Lab” in your email subject